Composite

Part:BBa_K1072012:Design

Designed by: Junqi kuang, Yaqing du and Junjie zhang   Group: iGEM13_SCUT   (2013-09-08)


Pgpa1+Chimeric Gpa1(Gα subunit)+Rfp+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2980
    Illegal AgeI site found at 3092
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 227


Design Notes

-


Source

Originating from C.elegan

References

1. Dowell SJ, Brown AJ.Yeast assays for G-protein-coupled receptors.Receptors Channels. 2002;8(5-6):343-52.

2. Brown AJ, Dyos SL, Whiteway MS, etd.Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein alpha-subunit chimeras.Yeast. 2000 Jan 15;16(1):11-22.